What is microparticle enzyme immunoassay?
Microparticle enzyme immunoassay. (MEIA) is a technique in which the solid-phase support consists of very small microparticles in liquid suspension. Specific reagent antibodies are covalently bound to the microparticles.
What is chemiluminescent microparticle immunoassay?
Chemiluminescent Microparticle Immuno Assay is the modified and advanced form of the Enzyme Linked Immuno Sorrbant Assay (ELISA) technique. Architect system is designed to detect antibodies to putative structural and non structural protein (HCr-43, c-100, NS3, NS4) of HCV genome [31].
What does chemiluminescence immunoassay methodology mean?
Chemiluminescence technology Chemiluminescent immunoassay (CLIA) is an immunoassay technique where the label, i.e. the true “indicator” of the analytic reaction, is a luminescent molecule.
What is the principle of immunoassays?
The principle behind the Immunoassay test is the use of an antibody that will specifically bind to the antigen of interest. The antibodies used in the Immunoassay must have a high affinity for the antigen. The antibodies used in the Immunoassay can either be monoclonal or polyclonal antibodies.
How does Cedia immunoassay work?
The CEDIA method is a single phase, competitive binding process that combines analyte-specific antibodies and two genetically engineered fragments of the bacterial enzyme ß-galactosidase to accurately and reliably detect the presence of drugs and their metabolites as well as other substances in serum, plasma, whole …
How does fluorescence polarization immunoassay work?
Fluorescence polarization immunoassay (FPA) is a homogeneous immunoassay useful for rapid and accurate detection of antibody or antigen. The principle of the assay is that a fluorescent dye (attached to an antigen or an antibody fragment) can be excited by plane-polarized light at the appropriate wavelength.
What is the principle of chemiluminescence?
Principle. Chemiluminescence relies on the reaction of NO with ozone. The chemical oxidation of NO by ozone yields nitrogen dioxide in an excited state. Relaxation from this excited state produces distinctive light emission (chemiluminescence) that is directly proportional to NO concentration.
What is the meaning of chemiluminescent?
Medical Definition of chemiluminescence : luminescence (as bioluminescence) due to chemical reaction usually at low temperatures.
What is the difference between ELISA and chemiluminescence?
Conclusion. CLIA and ELISA have a higher sensitivity compared with PA. CLIA has a high concordance with ELISA. Moreover, CLIA has a higher specificity and sensitivity for the detection of IgM and IgG and should be used for the clinical diagnosis of MP infection.
Why are immunoassays important?
Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical pharmacokinetic and bioequivalence studies in drug discovery and pharmaceutical industries.
What is Cedia in drug testing?
CEDIA (Cloned Enzyme Donor Immunoassay) for Screening Drugs of Abuse in Urine and the Effect of Adulterants.