What does TRITC stain for?

What does TRITC stain for?

Tetramethylrhodamine (TRITC) dashboard Tetramethylrhodamine is a traditional orange-fluorescent dye which has been used extensively to label protein conjugates such as phalloidins for cytoskeleton counterstaining or antibody conjugates for immunofluorescence.

Is Cy3 a TRITC?

Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.

Is Cy3 red or green?

Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).

What color is Cy5?

Cy5 dye is a traditional far-red–fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.

What Colour is rhodamine?

red
Rhodamine B

What is DAPI FITC and TRITC?

The DAPI-FITC-TRITC triple band fluorescence filter combination is designed specifically for simultaneous detection of the fluorochromes DAPI, FITC, and TRITC with minimal crossover (spectral bleed-through) between adjacent bands, and it can also be employed with other combinations of fluorescent probes that have …

What does Cy3 stand for?

Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λex=554, λem=568).

What is FAM fluorophore?

FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5′ or 3′ end of oligos.

How toxic is rhodamine?

For Rhodamine B, the effective and lethal concentration (EC50 and LC50) –causing 50% toxicity were in the range of 14–24 mg/L. For Rhodamine WT, no statistically significant effects were observed (p<0.05) at the tsted concentrations (up to 91, 100 and 200 mg/L for algae, crustaceans and fish embryos, respectively).

What is FITC in flow cytometry?

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.