What are BSL-2 conditions?

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BSL-2 laboratories are used to study moderate-risk infectious agents or toxins that pose a moderate danger if accidentally inhaled, swallowed, or exposed to the skin. Design requirements for BSL-2 laboratories include hand washing sinks, eye washing stations, and doors that close and lock automatically.

What are the requirements for a BSL-2 lab?

In a BSL-2 lab, the following conditions are to exist:

Doors. Doors that can be locked and secured should be installed for facilities that house restricted areas.
Public.
Sink.
Cleaning.
Bench Tops.
Lab Furniture.
Biological Safety Cabinets.
Eyewash Station.

What is the difference between BSL-2 and BSL 3?

This is provided to demonstrate that BSL-2 is largely achieved through sound lab safety practices and BSL-3 is achieved through the addition of facility features and equipment designed for containment of aerosols to a foundation of sound lab safety practices.

What is the difference between BSL-1 and BSL-2?

BSL-2 laboratories maintain the same standard microbial practices as BSL-1 labs, but also includes enhanced measures due to the potential risk of the aforementioned microbes.

Which of the following material’s must be handled at BSL-2?

Gloves and Eye Protection Gloves must be worn while working at the BSL2 level.

Which BSL does not require a primary barrier?

Biosafety Level 1 (BSL-1)
Biosafety Level 1 (BSL-1) BSL-1 applies to the basic level of containment and essentially represents good microbiological practice with no special primary or secondary barriers required.

What is the difference between BSL2 and BSL2+?

Biosafety Level 2+ (BSL2+) This biosafety level is also referred to as “Biosafety Level 2 Enhanced”. Work conducted at BSL2+ usually involves work with biological agents that would normally be conducted at BSL2; however, the work may involve certain conditions which would necessitate an increased amount of precautions.

What is the minimum PPE you should wear in the lab when working at BSL 2?

Wear appropriate PPE when working with infectious agents. Standard BSL-2 PPE consists of a dedicated lab coat, gloves, and eye protection. Other PPE may be required (see EH&S PPE Assessment Guide). Do not wear PPE in public areas.

What kind of work may need to be done in a PC2 containment facility?

Physical Containment Level 2 (PC2) If activities involving material which is likely to aerosolise, or with microorganisms transmissable by the respiratory route are performed, they must be done so in a biosafety cabinet (BSC).

What biosafety cabinet can be installed in a BSL 2 laboratory?

For Biosafety Level 2 applications involving toxic chemicals or radionuclides, a Class II- B type cabinet must be installed. Class II-B cabinets do not allow in-room venting of exhaust air and are thus appropriate for such uses.

What are two examples of secondary barriers in a BSL 3 lab?

Examples of secondary barriers include work areas that are separate from public areas, decontamination facilities, handwashing facilities, special ventilation systems, and airlocks.

What is the difference between a primary and secondary protective barrier?

Protective barrier: a) Primary = barrier sufficient to attenuate the useful beam to the required degree. b) Secondary = barrier sufficient to attenuate the stray radiation (leakage + scattered) to the required degree.