How does RetroNectin work?
RetroNectin reagent enhances transduction by facilitating colocalization of viral particles and hematopoietic cells; virus particles bind to the RetroNectin peptide via interaction with the heparin-binding domain, and hematopoietic cells bind mainly through the interaction of the cell surface integrin receptor VLA-4 …
How do you coat a plate with RetroNectin?
Coat a plate using 20 – 100 μg/ml RetroNectin with a volume corresponding to 4 – 20 μg/cm2 plate area. a 35 mm diameter dish (9 cm2), the concentration used for coating is 5 μg/cm2. Note: To avoid loss of RetroNectin fragment, do not filter-sterilize RetroNectin solution diluted with PBS.
What is Spinoculation?
In general, during spinoculation, cells are centrifuged at a low speed (approximately 1,000 to 2,000 × g) for several hours. This leads to a dramatic enhancement of viral replication of up to several-hundred-fold.
Where do Jurkat cells come from?
The Jurkat cell line is an immortalized T lymphocyte cell line that was originally obtained from the peripheral blood of a boy with T cell leukemia [14].
What is the purpose of transfection?
The main purpose of transfection is to study the function of genes or gene products, by enhancing or inhibiting specific gene expression in cells, and to produce recombinant proteins in mammalian cells [3].
What is transfection method?
Transfection is the process of introducing nucleic acids into eukaryotic cells by nonviral methods. Using various chemical or physical methods, this gene transfer technology enables the study of gene function and protein expression in a cellular environment.
What are Jurkat cells used for?
Jurkat cells are an immortalized line of T lymphocyte cells. And they are used to study acute T cell leukemia, T cell signaling, and the expression of a number of chemokine receptors susceptible to viral entry, particularly HIV.