What is scaling up in chromatography?
The process is scaled by increasing the column diameter while keeping the bed height , the velocity , and the volumes of the different phases (measured in column volumes) constant. The problem with this simple and well established concept , usually referred to as linear scale-up , is the lack of flexibility.
What are preparative columns?
The Preparative OBD Column is designed to incorporate a pair of specially-designed distributors and chemically-inert seals made to prevent leaks at high operating pressures.
What is the difference between analytical and preparative column?
“Preparative columns” are meant for isolating compounds from natural (product) extracts. It is meant for purifying compounds at a large scale, say in terms of ‘milligram’ or ‘gram’. “Analytical columns” are meant for qualitative analyses. The eluents from the analytical column may not have to be collected.
How does preparative chromatography work?
Preparative chromatography is a technique used for separating the ingredients of complex mixtures. It is used in the pharmaceutical industry to purify molecules by cleaning them of their impurities.
What is residence time in chromatography?
The residence time of a fluid parcel is the total time that the parcel has spent inside a control volume (e.g.: a chemical reactor, a lake, a human body).
How do you calculate column bed height?
The empty field is calculated using the formula: Bed volume (L) = Bed height (cm) * Column crossectional area (cm2) / 1000. Note that this calculation does not take into consideration the extra volume in the conical end cells which are present in some columns for example Chromaflow.
What is the length of preparative column in HPLC?
250 to 1,000 mm
Standard column dimensions range from 50 to 100 mm inner diameter and 250 to 1,000 mm in length.
What is the difference between analytical and preparative chromatography?
The main difference between preparative and analytical chromatography is that the main purpose of preparative chromatography is to isolate and purify a reasonable quantity of a specific substance from a sample whereas the main purpose of analytical chromatography is to separate the components of a sample.
What is preparative scale?
In analytical scale separations the quantity of samples injected is small, usually in micro liter levels whereas in preparative scale separations injection volumes are large so as to yield pure compounds in milligrams to gram levels.
What is a preparative method?
Precursor method involves atomic level mixing by forming a solid compound (precursor) in which the metals of the desired compound are present in the proper stoichiometry. For example, a mixed salt of an oxyacid (e.g. acetate) containing M and M’ in the ratio of 1:2 is formed in the case of formation of MM’2O4.
What is preparative thin layer chromatography?
Preparative thin layer chromatography (PTLC) is used to separate and isolate amounts of material larger than are normal for analytical TLC. The quantities processed range from 10 mg to greater than 1 gram.
What is the difference between space time and residence time?
space time is the time required to process the one reactor volume of feed and the residence time is the amount of time spent by a particle until it exits the reactor.