What does cyanine mean?
Definition of cyanine : any of various dyes used especially to sensitize photographic film to light from the green, yellow, red, and infrared regions of the spectrum.
What does Cy3 stain for?
The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiI and its variants.
What is the size of Cy3?
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission).
Is Cy5 far-red?
Invitrogen Cy5 dye is a bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines.
Is Cy3 a protein?
The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications.
Can you use APC and PE Cy5 together?
For example, PE-Cy™5 is very bright with regard to stain index (Table 1), but it has considerable spillover (ie, adds background) into the APC detector. While these two fluorochromes can be used together, the resolution sensitivity in APC will be reduced compared to, for example, a combination of PerCP-Cy5. 5 and APC.
What is the extinction coefficient for proteins?
Extinction coefficients for proteins are determined at absorbance maxima near 280 nm. In many bio-analytical applications, it is important to estimate or accurately determine the concentration of sample solutions containing purified biomolecules such as oligonucleotides, peptides or proteins.
What is the extinction coefficient of cysteine in disulfide bonds?
Cystine (C) in disulfide bonds has a relatively low extinction coefficient of 125 M-1cm-1. The absorbance of reduced cysteine is negligible at wavelength above 260 nm.
Why do Cyanines have a high extinction coefficient?
Due to their structure, cyanines have outstandingly high extinction coefficients often exceeding 100,000 Lmol -1cm -1. Different substituents allow to control properties of the chromophore, such as absorbance wavelength, photostability, and fluorescence.
Are Cy3 thermodynamic effects localized to oligonucleotide 5′ terminus?
This result suggests that Cy3 thermodynamic effects are localized to oligonucleotide 5′ terminus and do not extend more than 4 base pairs (half of the measured duplexes). Table 2. Additivity of stabilizing Gibbs energies (kcal.mol − 1) for DNA duplexes labeled with one or two Cy3 dyes. All thermodynamic values were experimentally measured.